Anti-CDX2 antibody [EPR2764Y]
| Name | Anti-CDX2 antibody [EPR2764Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76541 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR2764Y |
| Applications | WB FC ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rabbit, Human, Mouse, Rat |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDX2 aa 1-100 (N terminal) |
| Description | Rabbit Monoclonal |
| Gene | CDX2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilutionLanes 2 - 3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilutionLane 1 : HT29 lysateLane 2 : Colon cancer lysateLane 3 : Human small intestine lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27224_ab76541_1.jpg)
Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilutionLanes 2 - 3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilutionLane 1 : HT29 lysateLane 2 : Colon cancer lysateLane 3 : Human small intestine lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab76541 staining CDX2 in human colon cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry.
ab76541 staining CDX2 in Human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
ab76541 showing positive staining in Colonic adenocarcinoma tissue.
ab76541 showing positive staining in Normal colon tissue.
ab76541 showing negative staining in Skeletal muscle tissue.
ab76541 showing negative staining in Normal kidney tissue.
ab76541 showing negative staining in Normal brain tissue.
ab76541 showing negative staining in Normal brain tissue.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD
Product References
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