Anti-CEBP Alpha antibody [EP708Y]
| Name | Anti-CEBP Alpha antibody [EP708Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40761 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP708Y |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues in the N-term of human C/EBP alpha was used as immunogen |
| Description | Rabbit Monoclonal |
| Gene | CEBPA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27347_ab40761a.gif)
Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg
ab40761 (1/250) staining HeLa cells using immunofluorescence.
Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cellsSecondaryHRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27350_CEBP-Alpha-Primary-antibodies-ab40761-8.jpg)
Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cellsSecondaryHRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
CEBPA-dependent HK3 and KLF5 expression in primary AML and during AML - CEBPA-dependent HK3 and KLF5 expression in primary AML and during AML
Federzoni EA, Humbert M, Torbett BE, Behre G, Fey MF, Tschan MP. Sci Rep. 2014 Mar 3;4:4261.
Protein disulfide isomerase blocks CEBPA translation and is up-regulated during - Protein disulfide isomerase blocks CEBPA translation and is up-regulated during
Haefliger S, Klebig C, Schaubitzer K, Schardt J, Timchenko N, Mueller BU, Pabst T. Blood. 2011 Jun 2;117(22):5931-40.