Anti-CEBP Beta antibody [E299]

• Supplier: Abcam
• Catalog: ab32358
• Prices: $401.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: E299
• Applications: EMSA WB ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in the C terminus of Rat CEBPB
• Description: Rabbit Monoclonal
• Gene: CEBPB
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab32358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32358, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CEBP Beta antibody [E299] (ab32358) at 1/1000 dilution + PC12 cell lysate.

Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.

All lanes : Anti-CEBP Beta antibody [E299] (ab32358) at 1/500 dilutionLane 1 : 3T3 CellsLane 2 : MCF7 CellsLane 3 : PC12 CellsLane 4 : Rat Spleen LysateLane 5 : Rat Kidney LysateLane 6 : Rat Heart LysateLane 7 : Rat Brain LysateLane 8 : Mouse Spleen LysateLane 9 : Mouse Kidney LysateLane 10 : Mouse Heart LysateLane 11 : Mouse Brain Lysate

Product References

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