Anti-Cellular Apoptosis Susceptibility antibody
| Name | Anti-Cellular Apoptosis Susceptibility antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54674 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: LIGLFELPED DTIPDEEHFI DIEDTPGYQT AFSQLAFAGK KEHDPVGQMV NNPKIHLAQS LHKLSTACPG RVPSMVSTSL NAEALQYLQG YLQAASVTLL , corresponding to amino acids 872-972 of Human Cellular Apoptosis Susceptibility Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CSE1L |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Cellular Apoptosis Susceptibility antibody (ab54674) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
ab54674 at 10 µg/ml staining Cellular Apoptosis Susceptibility human Hela cells by Immunocytochemistry / Immunofluorescence.
All lanes : Anti-Cellular Apoptosis Susceptibility antibody (ab54674) at 1/2500 dilutionLane 1 : Hela extract 25 ugLane 2 : RPE-1 extract 25 ugLane 3 : Hela extract 50 ugLane 4 : RPE-1 extract 25 ugSecondaryDonkey LICOR anti-mouse IRDye680 at 1/20000 dilutionPerformed under reducing conditions.
![Overlay histogram showing HeLa cells stained with ab54674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54674, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/27478_Cellular-Apoptosis-Susceptibility-Primary-antibodies-ab54674-7.jpg)
Overlay histogram showing HeLa cells stained with ab54674 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54674, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.