Anti-CEP55 antibody [EPR11944(B)]

• Supplier: Abcam
• Catalog: ab170414
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11944(B)
• Applications: IHC-P WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CEP55
• Description: Rabbit Monoclonal
• Gene: CEP55
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/5000 dilution + SW480 Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

All lanes : Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution (unpurified)Lane 1 : SW480 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysatedeveloped using the ECL techniquePerformed under reducing conditions.