Anti-CERK antibody
| Name | Anti-CERK antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38011 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Hamster, Human, Hamster |
| Antigen | Synthetic peptide corresponding to Human CERK aa 487-516 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | CERK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab38011 stained SKNSH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38011, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CERK antibody (ab38011) at 1/100 dilutionLane 1 : Mouse heart tissue lysate Lane 2 : A2058 cell lysate