CERK

Gene Symbol CERK
Entrez Gene 64781
Alt Symbol LK4, dA59H18.2, dA59H18.3, hCERK
Species Human
Gene Type protein-coding
Description ceramide kinase
Other Description acylsphingosine kinase|lipid kinase 4|lipid kinase LK4
Swissprots Q9UGE5 Q8TCT0 Q6NX59 A8K611 Q9BYB3 A0JNT4
Accessions EAW73438 EAW73439 EAW73440 EAW73441 EAW73442 Q8TCT0 AB051433 BAB33316 AB079066 BAC01154 AJ457828 CAD29884 AK025083 AK026892 BAB15585 AK291476 BAF84165 AL137600 AL832794 BC004278 AAH04278 BC008382 BC067255 AAH67255 BC108712 AAI08713 BC126940 AAI26941 BQ710170 BU187541 CR456404 CAG30290 CR457365 CAG33646 CU012949 CAK54380 CU013237 CAK54679 XM_011530315 XP_011528617 NM_182661 NM_022766 NP_073603
Function Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. {ECO:0000269|PubMed:19168031}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:19168031}. Membrane {ECO:0000269|PubMed:19168031}; Peripheral membrane protein {ECO:0000269|PubMed:19168031}.
Tissue Specificity High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
Top Pathways Sphingolipid metabolism

Ceramide Kinase (G-3) - sc-376730 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ceramide Kinase (G-3): sc-376730. Western blot analysis of Ceramide Kinase expression in non-transfected: sc-117752 (A) and human Ceramide Kinase transfected: sc-128306 (B) 293T whole cell lysates.

Ceramide Kinase (H-237) - sc-98700 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ceramide Kinase (H-237): sc-98700. Western blot analysis of Ceramide Kinase expression in mouse heart tissue extract.

Ceramide Kinase (Y-20) - sc-79409 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Ceramide Kinase (C-20) - sc-79406 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CERK antibody - ab38011 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab38011 stained SKNSH cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38011, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CERK antibody - ab155061 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CERK antibody (ab155061) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-Ceramide Kinase / CERK Antibody (Internal, DY550) - LS-C141083 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (aa495-524, APC) - LS-C221742 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody - LS-C152969 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (aa495-524, FITC) - LS-C221743 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (aa495-524, AP) - LS-C221741 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (aa495-524, PE) - LS-C221745 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (C-Terminus) - LS-C151633 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Ceramide Kinase / CERK antibody Western Blot of Fetal Brain.

Anti-Ceramide Kinase / CERK Antibody (aa50-150) - LS-C49067 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Detection of Ceramide Kinase in A549 cell lysate.

Anti-Ceramide Kinase / CERK Antibody - LS-C306910 from LifeSpan Bioscience

Host Mouse
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (aa495-524) - LS-C329370 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (Internal) - LS-C140575 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CERK staining of formalin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 ug/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Anti-Ceramide Kinase / CERK Antibody (N-Terminus) - LS-C140576 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CERK staining of formalin fixed paraffin embedded human brain (cortex) tissue at a dilution of 20 ug/ml. Antigen retrieval using a citrate buffer and steam/heat was utilized.

Anti-Ceramide Kinase / CERK Antibody (aa449-498) - LS-C205845 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Ceramide Kinase / CERK antibody Western Blot of 721_B. Antibody dilution: 1 ug/ml.

Anti-Ceramide Kinase / CERK Antibody (aa487-516) IHC-plus™ - LS-B9627 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-Ceramide Kinase / CERK antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9627 dilution 1:50.

Anti-Ceramide Kinase / CERK Antibody (Internal) IHC-plus™ - LS-A4556 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CERK antibody LS-A4556 IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-Ceramide Kinase / CERK Antibody (N-Terminus) IHC-plus™ - LS-A3369 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CERK antibody LS-A3369 IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-Ceramide Kinase / CERK Antibody - LS-C26838 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot of Ceramide Kinase antibody (X1705P) on 30 ng of recombinant ceramide kinase. Antibody used at 2 ug/ml. Visualized using Pierce West Femto substrate system. Secondary used at 1:75k dilution. Exposure for 60 seconds.

Anti-Ceramide Kinase / CERK Antibody - LS-C26841 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-Ceramide Kinase / CERK Antibody (Internal, Biotin) - LS-C141079 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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