All lanes : Anti-cGKI antibody [9A4] (ab119000) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY cGKI cDNALysates/proteins at 5 µg per lane.
ab119000 at 1/100 dilution staining cGKI in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
ab119000 at 1/100 dilution staining cGKI in COS7 cells transiently transfected by pCMV6-ENTRY cGKI by Immunofluorescence.
ab119000 at 1/100 dilution staining cGKI in HEK293T cells transfected with either cGKI overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry.