Western blot detection of Cdk5 phosphorylation in untreated (A,C) and EGF-treated A-431 cultures (B,D). Blots were probed with Cdk5 (J-3): sc-6247 (A,B) and p-Cdk5 (Tyr 15): sc-12918 (C,D).
Western blot analysis of Cdk5 phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase treated (C,F) SH-SY5Y whole cell lysates. Antibodies tested include p-Cdk5 (Tyr 15)-R: sc-12918-R (A,B,C) and Cdk5 (C-8): sc-173 (D,E,F).
Western blot analysis of Cdk5 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) SK-N-MC whole cell lysates. Antibodies tested include p-Cdk5 (Tyr 15)-R: sc-12918-R (A,B) and Cdk5 (J-3): sc-6247 (C,D).