CDK5

Gene Symbol CDK5
Entrez Gene 1020
Alt Symbol PSSALRE
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase 5
Other Description TPKII catalytic subunit|cell division protein kinase 5|cyclin-dependent-like kinase 5|protein kinase CDK5 splicing|serine/threonine-protein kinase PSSALRE|tau protein kinase II catalytic subunit
Swissprots A1XKG3 Q00535
Accessions EAL24498 EAW54049 EAW54050 EAW54051 Q00535 AK026533 AY049778 AAL15435 AY927560 BC005115 AAH05115 BP309068 BT006680 AAP35326 BU527089 CA429180 CB114175 CR457041 CAG33322 DB487008 DQ411039 ABD66016 L04658 X66364 CAA47007 NM_001164410 NP_001157882 NM_004935 NP_004926
Function Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3- type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many
Subcellular Location Isoform 1: Cytoplasm. Cell membrane; Peripheral membrane protein. Perikaryon. Cell projection, lamellipodium {ECO:0000250}. Cell projection, growth cone {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Note=In axonal growth cone with extension to the peripheral lamellipodia (By similarity). Under neurotoxic stress and neuronal injury conditions, CDK5R (p35) is cleaved by calpain to generate CDK5R1 (p25) in response to increased intracellular calcium. The elevated level of p25, when in complex with CDK5, leads to its subcellular misallocation as well as its hyperactivation. Colocalizes with CTNND2 in the cell body of neuronal cells, and with CTNNB1 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Reversibly attached to the plasma membrane in an inactive form when complexed to dephosphorylated p35 or CDK5R2 (p39), p35 phosphorylation releases this attachment and activates CDK5.
Tissue Specificity Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary. {ECO:0000269|PubMed:17009320, ECO:0000269|PubMed:19693690}.
Top Pathways Axon guidance, Alzheimer's disease, Cocaine addiction

CDK5 (1H3) Mouse mAb - 12134 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1H3
Western blot analysis of extracts from NIH/3T3, KNRK, and COS-7 cells using CDK5 (1H3) Mouse mAb.

CDK5 (D1F7M) Rabbit mAb - 14145 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1F7M
Western blot analysis of extracts from HeLa cells expressing either shCDK5 or non-targeting shRNA (shNT), using CDK5 (D1F7M) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

CDK5 Antibody - 2506 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from PC12 and Jurkat cells, using CDK5 Antibody.

Cdk5 (C-8) - sc-173 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk5 (C-8): sc-173. Western blot analysis of Cdk5 expression in HeLa (A), K-562 (B), Jurkat (C) and SK-BR-3 (D) phorbol ester-induced nuclear extracts.

Cdk5 (J-3) - sc-6247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk5 (J-3): sc-6247. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Cdk5 (DC 17) - sc-249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Cdk5 (DC 17): sc-249. Western blot analysis of Cdk5 expression in HeLa (A), K-562 (B), Jurkat (C) and SK-BR-3 (D) nuclear extracts.

Cdk5 (H-291) - sc-750 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Cdk5 (H-291): sc-750. Western blot analysis of Cdk5 expression in Jurkat nuclear extract.

Cdk5 (G-10) - sc-32497 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Cdk5 (C-7) - sc-377558 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Cdk5 phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) SK-N-MC whole cell lysates. Antibodies tested include p-Cdk5 (C-7): sc-377558 (A,B) and Cdk5 (J-3): sc-6247 (C,D).

p-Cdk5 (Tyr 15) - sc-12918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot detection of Cdk5 phosphorylation in untreated (A,C) and EGF-treated A-431 cultures (B,D). Blots were probed with Cdk5 (J-3): sc-6247 (A,B) and p-Cdk5 (Tyr 15): sc-12918 (C,D).

p-Cdk5 (Ser 159) - sc-12919 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Cdk5 (Ser 159)-R: sc-12919-R. Western blot analysis of Cdk5 phosphorylation in untreated (A), nocodazole treated (B) and nocodazole and lambda protein phosphatase (sc-200312A) treated (C) SK-N-SH whole cell lysates.

Anti-Cdk5 (phospho Y15) antibody [EP762RY] - ab53279 from Abcam

Host Rabbit
Clonality Monoclonal
Clone EP762RY
Lanes 1 - 2 : Pan Cdk5Lanes 3 - 4 : Anti-Cdk5 (phospho Y15) antibody [EP762RY] (ab53279) at 1/2000 dilutionLane 1 : HeLa cell lysate treated with APLane 2 : HeLa cell lysateLane 3 : HeLa cell lysate treated with APLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-Cdk5 antibody - ab196962 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk5 antibody (ab196962) at 1/500 dilutionLane 1 : COS7 cell extract with synthesized peptideLane 2 : COS7 cell extract

Anti-Cdk5 antibody - ab118860 from Abcam

Host Rabbit
Clonality Polyclonal
ab118860, at 1/500 dilution, staining Cdk5 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Cdk5 antibody - ab21249 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Standard Curve for Cdk5 (Analyte: Cdk5 protein (Tagged) (ab56282)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2G2] to Cdk5 (ab28441) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk5 (ab21249) at 0.5µg/ml.

Anti-Cdk5 antibody - ab189870 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Cdk5 antibody (ab189870) at 1/500 dilutionLane 1 : 293 cell lysateLane 2 : HepG2 cell lysateLane 3 : BT474 cell lysateLane 4 : Mouse brain lysateLane 5 : Mouse spinal cord lysate

Anti-Cdk5 antibody - ab6543 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Cdk5 antibody - ab88837 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Cdk5 antibody (ab88837) at 1 µg/ml + rat brain tissue lysate at 50 µgdeveloped using the ECL technique

Anti-Cdk5 antibody [2G2] - ab28441 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G2
Standard Curve for Cdk5 (Analyte: Cdk5 protein (Tagged) (ab56282)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2G2] to Cdk5 (ab28441) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk5 (ab21249) at 0.5µg/ml.

Anti-Cdk5 antibody [4E4] - ab115812 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E4
ELISA showing a serial dilution of ab115812.

Anti-Cdk5 antibody [8A1] - ab28438 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8A1
All lanes : Anti-Cdk5 antibody [8A1] (ab28438) at 1/1000 dilutionLane 1 : SH-SY5Y cell lysatesLane 2 : SK-N-SH cell lysatesLane 3 : Mouse brain tissues

Anti-Cdk5 antibody [DC17 + DC34] - ab2362 from Abcam

Prices $370.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DC17 + DC34

Anti-Cdk5 antibody [DC17 + DC34] - BSA and Azide free - ab77673 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone DC17 + DC34

Anti-Cdk5 antibody [EP715Y] - ab40773 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP715Y
developed using the ECL techniquePerformed under reducing conditions.

Anti-Cdk5 antibody [EP715Y] (Alexa Fluor® 488) - ab199312 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP715Y
ab199312 staining Cdk5 in SKNSH cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199312 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Search more