Anti-CHD1L antibody [2170C3a]

• Supplier: Abcam
• Catalog: ab51324
• Prices: $395.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2170C3a
• Applications: WB IP DB IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Recombinant human CHD1L fragment
• Description: Mouse Monoclonal
• Gene: CHD1L
• Conjugate: Unconjugated

Product images

Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique

Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.

IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Clinical significance of CHD1L in hepatocellular carcinoma and therapeutic - Clinical significance of CHD1L in hepatocellular carcinoma and therapeutic

Chen L, Yuan YF, Li Y, Chan TH, Zheng BJ, Huang J, Guan XY. Gut. 2011 Apr;60(4):534-43.

CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is - CHD1L promotes hepatocellular carcinoma progression and metastasis in mice and is

Chen L, Chan TH, Yuan YF, Hu L, Huang J, Ma S, Wang J, Dong SS, Tang KH, Xie D, Li Y, Guan XY. J Clin Invest. 2010 Apr;120(4):1178-91.