CHD1L

Gene Symbol	CHD1L
Entrez Gene	9557
Alt Symbol	ALC1, CHDL
Species	Human
Gene Type	protein-coding
Description	chromodomain helicase DNA binding protein 1-like
Other Description	amplified in liver cancer 1\|amplified in liver cancer protein 1\|chromodomain-helicase-DNA-binding protein 1-like
Swissprots	Q96HF7 Q6PK83 A5YM64 B5MDZ7 Q96SP3 Q86XH3 B4DDE1 Q53EZ3 Q9NVV8 Q86WJ1 Q5VXX7 Q9BVJ1 Q6DD94
Accessions	CAH72650 EAW50936 EAW50937 EAW50938 Q86WJ1 AF054177 AAC39911 AF537213 AAO49505 AK001342 BAA91637 AK026183 BAB15386 AK027631 BAB55248 AK223496 BAD97216 AK293157 BAG56702 AK297135 BAG59638 AK298424 BAG60647 AK307400 BC001171 AAH01171 BC005038 AAH05038 BC008649 AAH08649 BC043501 AAH43501 BC053354 BC077717 AAH77717 BG195857 CB153921 CN255647 CN255649 CN255654 CR749729 DA007597 DA155685 DA745071 EC452903 EF560738 ABQ59048 KJ534809 AHW56449 R46126 XM_006711638 XP_006711701 XM_006711639 XP_006711702 XR_922001 NM_001256336 NP_001243265 NM_001256337 NP_001243266 NM_001256338 NP_001243267 NM_004284 NP_004275 NM_024568 NP_078844 NR_046070
Function	DNA helicase which plays a role in chromatin-remodeling following DNA damage. Targeted to sites of DNA damage through interaction with poly(ADP-ribose) and functions to regulate chromatin during DNA repair. Able to catalyze nucleosome sliding in an ATP-dependent manner. Helicase activity is strongly stimulated upon poly(ADP-ribose)-binding. {ECO:0000269\|PubMed:18023026, ECO:0000269\|PubMed:19661379}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:19661379}. Note=Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1.
Tissue Specificity	Frequently overexpressed in hepatomacellular carcinomas. {ECO:0000269\|PubMed:18023026}.

CHD1L (E1I8C) Rabbit mAb - 13460 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	E1I8C

Western blot analysis of extracts from various cell lines using CHD1L (E1I8C) Rabbit mAb.

CHD1L (P-17) - sc-104137 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

CHD1L (P-17): sc-104137. Western blot analysis of CHD1L expression in U-937 (A) and Hep G2 (B) nuclear extracts.

CHD1L (H-88) - sc-98309 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

CHD1L (H-88): sc-98309. Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.

CHD1L (2170C3a) - sc-81065 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

CHD1L (2170C3a): sc-81065 Western Blot analysis of human recombinant CHD1L fusion protein.

Anti-CHD1L antibody - ab118799 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Detection of CHD1L in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118799 at 6 µg/mg lysate for IP (Lane 1). For WB detection an anti-CHD1L antibody which recognizes an upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-CHD1L antibody [2170C3a] - ab51324 from Abcam

Prices	$395.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	2170C3a

Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µgSecondaryanti-mouse IgG at 1/2500 dilutiondeveloped using the ECL technique

Anti-CHD1L antibody [EPR14515(2)] - ab197019 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR14515(2)

All lanes : Anti-CHD1L antibody [EPR14515(2)] (ab197019) at 1/5000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : A549 (Human lung carcinoma) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CHD1L Antibody (aa560-590, FITC) - LS-C233786 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590, PE) - LS-C233787 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590, Azide-free, HRP) - LS-C249664 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa550-600) - LS-C290027 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa545-594) - LS-C199812 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from HepG2 cells, using CHD1L antibody.

Anti-CHD1L Antibody (clone 2170C3a) - LS-C184366 from LifeSpan Bioscience

Prices	$335.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal
Clone	2170C3a

Western blot of immunized recombinant protein using CHD1L antibody. Left: CHD1L staining. Right: Coomassie Blue staining of immunized recombinant protein.

Anti-CHD1L Antibody (aa847-897) - LS-C290028 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590) - LS-C168725 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

CHD1L Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CHD1L antibody detected the CHD1L protein (arrow).

Anti-CHD1L Antibody (aa560-590) - LS-C320348 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (clone 2170C3a) - LS-C342417 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2170C3a

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CHD1L mAb.

Anti-CHD1L Antibody - LS-C79100 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Mouse
Clonality	Monoclonal

Anti-CHD1L Antibody (aa759-879) - LS-C182811 from LifeSpan Bioscience

Prices	$650.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590, AP) - LS-C233782 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590, APC) - LS-C233783 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa560-590, Biotin) - LS-C233785 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-CHD1L Antibody (aa62-111) - LS-C31613 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

CHD1L Antibody - NBP1-89153 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

CHD1L Antibody - AF6959 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CHD1L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1L at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Search more