All lanes : Anti-CHD3 antibody (ab176804) at 0.1 µg/mlLane 1 : HeLa cell lysate at 50 µgLane 2 : HeLa cell lysate at 15 µgLane 3 : 293T cell lysate at 50 µgLane 4 : Jurkat cell lysate at 50 µgdeveloped using the ECL technique
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). 1) and 2) different lots of ab176804 used at 6 µg/mg lysate. 3) control IgG. For blotting ab176804 was used at 1 µg/ml. Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate carcinoma tissue, labeling CHD3 using ab176804 at a 1/1000 dilution, followed by DAB staining.