Anti-CHD4 antibody
| Name | Anti-CHD4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54603 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | FC WB IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: ETEPKGAADV EKVEEKSAID LTPIVVEDKE EKKEEEEKKE VMLQNGETPK DLNDEKQKKN IKQRFMFNIA DGGFTELHSL WQNEERAATV TKKTYEIWH, corresponding to amino acids 1632-1731 of Human CHD4 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CHD4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
CHD4 antibody (ab54603) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human transitional cell carcinoma.
![Overlay histogram showing HeLa cells stained with ab54603 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54603, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/28195_CHD4-Primary-antibodies-ab54603-1.jpg)
Overlay histogram showing HeLa cells stained with ab54603 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54603, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG2a [ICIGG2A], (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
A new non-catalytic role for ubiquitin ligase RNF8 in unfolding higher-order - A new non-catalytic role for ubiquitin ligase RNF8 in unfolding higher-order
Luijsterburg MS, Acs K, Ackermann L, Wiegant WW, Bekker-Jensen S, Larsen DH, Khanna KK, van Attikum H, Mailand N, Dantuma NP. EMBO J. 2012 May 30;31(11):2511-27.
The NuRD chromatin-remodeling complex regulates signaling and repair of DNA - The NuRD chromatin-remodeling complex regulates signaling and repair of DNA
Smeenk G, Wiegant WW, Vrolijk H, Solari AP, Pastink A, van Attikum H. J Cell Biol. 2010 Sep 6;190(5):741-9.
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Burd CJ, Kinyamu HK, Miller FW, Archer TK. J Biol Chem. 2008 Dec 12;283(50):34976-82.