Anti-CHD4 antibody [3F2/4] - ChIP Grade
| Name | Anti-CHD4 antibody [3F2/4] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab70469 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3F2/4 |
| Applications | ICC/IF ICC/IF ChIP ELISA WB IP IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1 |
| Description | Mouse Monoclonal |
| Gene | CHD4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab70469 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70469, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab70469 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HeLa cells stained with ab70469 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70469, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/28202_CHD4-Primary-antibodies-ab70469-3.jpg)
Overlay histogram showing HeLa cells stained with ab70469 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70469, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
CHD4 immunoprecipitation from murine brain tissue lysate using ab70469. 5000 µg of cell lysate was incubated with primary antibody (undiluted) and matrix (protein G) and incubated for 14 hours. An HRP-conjugated rabbit anti-CHD4 polyclonal (1/1000) was used to detect CHD4 immunoprecipitation.See Abreview
Product References
Analysis of the SWI/SNF chromatin-remodeling complex during early heart - Analysis of the SWI/SNF chromatin-remodeling complex during early heart
Singh AP, Archer TK. Nucleic Acids Res. 2014 Mar;42(5):2958-75.
The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of - The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of
Sparmann A, Xie Y, Verhoeven E, Vermeulen M, Lancini C, Gargiulo G, Hulsman D, Mann M, Knoblich JA, van Lohuizen M. EMBO J. 2013 May 29;32(11):1598-612.
The NuRD chromatin-remodeling enzyme CHD4 promotes embryonic vascular integrity - The NuRD chromatin-remodeling enzyme CHD4 promotes embryonic vascular integrity
Ingram KG, Curtis CD, Silasi-Mansat R, Lupu F, Griffin CT. PLoS Genet. 2013;9(12):e1004031.
The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular - The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular
Curtis CD, Griffin CT. Mol Cell Biol. 2012 Apr;32(7):1312-20.
Mi-2/NuRD is required in renal progenitor cells during embryonic kidney - Mi-2/NuRD is required in renal progenitor cells during embryonic kidney
Denner DR, Rauchman M. Dev Biol. 2013 Mar 15;375(2):105-16.