ICC/IF image of ab103153 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103153 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Chemerin antibody (ab103153) at 1/500 dilution + Mouse testis lysate at 50 µg
All lanes : Anti-Chemerin antibody (ab103153) at 1/500 dilutionLane 1 : Chemerin transfected 293T cell line lysateLane 2 : Non-transfected 293T cell lineLysates/proteins at 25 µg per lane.