Anti-CHFR antibody
| Name | Anti-CHFR antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4184 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CHFR |
| Description | Rabbit Polyclonal |
| Gene | CHFR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluoresence using ab4184 on SAOS2 cells with a FITC conjugated secondary antibody. The foci seen are confined to the nuclei of the cells present in the picture.
All lanes : Anti-CHFR antibody (ab4184) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of ab4184 staining in Human Breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4184, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
USP7 and Daxx regulate mitosis progression and taxane sensitivity by affecting - USP7 and Daxx regulate mitosis progression and taxane sensitivity by affecting
Giovinazzi S, Morozov VM, Summers MK, Reinhold WC, Ishov AM. Cell Death Differ. 2013 May;20(5):721-31.
Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. - Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins.
Ahel I, Ahel D, Matsusaka T, Clark AJ, Pines J, Boulton SJ, West SC. Nature. 2008 Jan 3;451(7174):81-5.