CHFR

Gene Symbol CHFR
Entrez Gene 55743
Alt Symbol RNF116, RNF196
Species Human
Gene Type protein-coding
Description checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase CHFR|RING finger protein 196
Swissprots Q9NVD5 Q9NRT4 B4DZ77 B4E2L6 Q9NT32 Q96SL3 Q96EP1 A6NEN5
Accessions EAW54809 EAW54810 EAW54811 EAW54812 EAW54813 EAW54814 EAW54815 Q96EP1 AF170724 AAF91084 AK001658 BAA91817 AK027687 BAB55297 AK054917 AK090948 AK097671 BAG53509 AK302785 BAG63989 AK304333 BAG65178 AL137561 CAB70812 BC012072 AAH12072 BP313700 DQ599878 DQ892232 ABM83158 DQ895433 ABM86359 NM_001161344 NP_001154816 NM_001161345 NP_001154817 NM_001161346 NP_001154818 NM_001161347 NP_001154819 NM_018223 NP_060693
Function E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. {
Subcellular Location Nucleus, PML body {ECO:0000269|PubMed:11912157, ECO:0000269|PubMed:15467728, ECO:0000269|PubMed:18172500}.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:10935642}.

CHFR (D40H6) Rabbit mAb - 6904 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D40H6
Western blot analysis of MDA-MB-231 cells untreated (-) or nocodazole-treated (+) using CHFR (D40H6) Rabbit mAb.

CHFR Antibody - 4297 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using CHFR Antibody.

Chfr (H-300) - sc-28263 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Chfr (H-300): sc-28263. Western blot analysis of Chfr expression in ES-2 whole cell lysate.

Chfr (C-16) - sc-13290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Chfr (N-15) - sc-13287 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Chfr (N-15): sc-13287. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells.

Chfr (D-15) - sc-13288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Chfr (D-15): sc-13288. Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear staining of subset of hematopoietic cells.

Chfr (L2) - sc-81832 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Chfr (L2): sc-81832. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human ovary, clear cell carcinoma tissue showing nuclear localization.

Anti-CHFR antibody - ab4184 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluoresence using ab4184 on SAOS2 cells with a FITC conjugated secondary antibody. The foci seen are confined to the nuclei of the cells present in the picture.

Anti-CHFR antibody - ab13773 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab13773 (2.5 µg/ml) staining CHFR in Human Brain (Cerebellum) by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows stained Purkinje cells in Cerebellum.

Anti-CHFR antibody - ab77225 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-CHFR antibody (ab77225) at 5 µg/ml + immunogen at 0.2 µgSecondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilutiondeveloped using the ECL technique

Anti-CHFR Antibody (clone 1H3-A12) - LS-C133778 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1H3-A12
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]

Anti-CHFR Antibody (aa36-85) IHC-plus™ - LS-B5739 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CHFR antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5739 concentration 2.5 ug/ml.

Anti-CHFR Antibody (aa475-504, AP) - LS-C254726 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa475-504, Biotin) - LS-C254727 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa475-504, FITC) - LS-C254729 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa475-504, APC) - LS-C254728 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa475-504, PE) - LS-C254731 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa475-504, Azide-free, HRP) - LS-C254730 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (aa1-653, Azide-free) - LS-C327062 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CHFR Antibody (aa475-504) - LS-C320350 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHFR Antibody (C-Terminus) - LS-C54652 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (2.5 ug/ml) staining of paraffin embedded human brain (cerebellum). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-CHFR Antibody (aa475-504) - LS-C168728 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CHFR Antibody western blot of A549 cell line lysates (35 ug/lane). The CHFR antibody detected the CHFR protein (arrow).

CHFR Antibody (1H3-A12) - H00055743-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1H3-A12
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.

CHFR Antibody - NBP1-52955 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

CHFR Antibody - NB100-1166 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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