All lanes : Anti-CHMP1a antibody [EPR11939] (ab178686) at 1/1000 dilutionLane 1 : HeLa cell lysatesLane 2 : 293T cell lysatesLane 3 : MCF-7 cell lysatesLysates/proteins at 10 µg per lane.
Flow cytometric analysis of permeabilized 293T cells using ab178686 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 cell lysate or (Lane 2) 1XPBS (negative control) using ab178686 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.