Anti-CHMP2B antibody
| Name | Anti-CHMP2B antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33174 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Chicken |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human CHMP2B |
| Description | Rabbit Polyclonal |
| Gene | CHMP2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-CHMP2B antibody (ab33174) at 1 µg/ml + Tagged recombinant CHMP2B protein at 0.1 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab33174 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33174, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab33174 staining CHMP2B in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. The antibody produced a diffuse and widespread staining in many brain areas. The image shows the staining obtained in the mouse hippocampus.See Abreview
ab33174 staining CHMP2B in human hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 4% serum for 1 hour at 27°C followed by incubation with the primary antibody at a 1/600 dilution for 18 hours at 4°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/500 dilution.See Abreview
Product References
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Charged multivesicular body protein 2B (CHMP2B) of the endosomal sorting complex - Charged multivesicular body protein 2B (CHMP2B) of the endosomal sorting complex
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