Anti-Choline Acetyltransferase antibody [EPR13024(B)] (HRP)

• Supplier: Abcam
• Catalog: ab193095
• Prices: $425.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13024(B)
• Applications: IHC-P WB
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Choline Acetyltransferase aa 700 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CHAT
• Conjugate: HRP

Product images

IHC image of Choline Acetyltransferase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193095 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC image of Choline Acetyltransferase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193095 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Choline Acetyltransferase antibody [EPR13024(B)] (HRP) (ab193095) at 1/2000 dilutionLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 25 µgLane 2 : Brain (Human) Tissue Lysate - fetal normal tissue at 20 µgdeveloped using the ECL techniquePerformed under reducing conditions.