CHAT

Gene Symbol CHAT
Entrez Gene 1103
Alt Symbol CHOACTASE, CMS1A, CMS1A2
Species Human
Gene Type protein-coding
Description choline O-acetyltransferase
Other Description acetyl CoA:choline O-acetyltransferase|choline acetylase
Swissprots Q9BQ35 Q9BQ23 Q9BQE1 A2BDF5 P28329 Q16488 A2BDF4
Accessions AAC37590 AAK08950 AAK08951 AAK08952 CAA39923 CAA40201 CAA46157 EAW93086 EAW93087 EAW93088 EAW93089 EAW93090 EAW93091 EAW93092 P28329 AF305907 AAK08953 AF305908 AAK08954 AF305909 AAK08955 AF450452 AAL48258 BC130615 AAI30616 BC130617 AAI30618 D82339 BAA18943 D82340 BAA18944 D82341 BAA18945 D82342 BAA18946 HQ258314 ADR83068 S45018 AAB23557 S56138 AAA14245 NM_001142929 NP_001136401 NM_001142933 NP_001136405 NM_001142934 NP_001136406 NM_020549 NP_065574 NM_020984 NP_066264 NM_020985 NP_066265 NM_020986 NP_066266
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Top Pathways Glycerophospholipid metabolism

choactase (D-16) - sc-19056 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
choactase (D-16): sc-19056. Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.

choactase (E-7) - sc-55557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
choactase (E-7): sc-55557. Western blot analysis of choactase expression in IMR-32 whole cell lysate.

choactase (H-95) - sc-20672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
choactase (H-95): sc-20672. Western blot analysis of choactase expression in IMR-32 (A) and SK-N-SH (B) whole cell lysates.

choactase (C-14) - sc-19057 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
choactase (C-14): sc-19057. Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.

Anti-Choline Acetyltransferase antibody - ab101755 from Abcam

Prices $379.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
ICC/IF image of ab101755 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101755 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Donkey anti-Goat IgG (H+L) (ab96931) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Choline Acetyltransferase antibody - ab34419 from Abcam

Prices $379.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal
This image shows a section of a formalin-fixed paraffin-embedded adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Antibody ab34419 at a dilution of 1/100.

Anti-Choline Acetyltransferase antibody - ab137349 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Choline Acetyltransferase antibody (ab137349) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-Choline Acetyltransferase antibody - ab134021 from Abcam

Prices $376.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Immunohistochemical analysis of a section of adult mouse brain labelling Choline Acetyltransferase (red staining) using ab134021 at 1/1000 dilution in cholinergic neurons of the caudate-putamen nucleus. The pale green staining is autofluorescence of green fluorescent protein (GFP) in this transgenic animal.

Anti-Choline Acetyltransferase antibody - ab74842 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-Choline Acetyltransferase antibody - ab27484 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Choline Acetyltransferase antibody (ab27484) at 0.03 µg/ml + Human placenta lysate at 35 µg

Anti-Choline Acetyltransferase antibody - ab54599 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab54599 Choline Acetyltransferase antibody at 1ug/ml.

Anti-Choline Acetyltransferase antibody [38B12] - ab49382 from Abcam

Host Mouse
Clonality Monoclonal
Clone 38B12
Overlay histogram showing SHSY-5Y cells stained with ab49382 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab49382, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Choline Acetyltransferase antibody [EPR13024(B)] - ab181023 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13024(B)
All lanes : Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023) at 1/5000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Alexa Fluor® 488) - ab192465 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13024(B)
ab192465 staining Choline Acetyltransferase in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192465 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Alexa Fluor® 647) - ab192466 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13024(B)
ab192466 staining Choline Acetyltransferase in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192466 at a working dilution of 1 in 100 (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed SKNSH cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Choline Acetyltransferase antibody [EPR13024(B)] (HRP) - ab193095 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13024(B)
IHC image of Choline Acetyltransferase staining in a section of formalin-fixed paraffin-embedded normal human cerebellum tissue, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193095 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CHAT Antibody - LS-C6078 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal

Anti-CHAT Antibody - LS-C6079 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CHAT Antibody - LS-C6077 from LifeSpan Bioscience

Prices $475.00
Sizes 50 µl
Host Goat
Clonality Polyclonal

Anti-CHAT Antibody - LS-C6076 from LifeSpan Bioscience

Prices $515.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-CHAT Antibody (clone 1.B3.9B3) - LS-C35920 from LifeSpan Bioscience

Prices $560.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1.B3.9B3

Anti-CHAT Antibody (clone 1E6) - LS-C41821 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E6

Anti-CHAT Antibody (aa167-188) - LS-C79271 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CHAT Antibody - LS-C79273 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Chicken
Clonality Polyclonal

Anti-CHAT Antibody - LS-C93992 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Sheep
Clonality Polyclonal
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