ICC/IF image of ab103369 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103369 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CHRDL1 antibody (ab103369) at 1/500 dilution + Mouse kidney tissue lysate
All lanes : Anti-CHRDL1 antibody (ab103369) at 1/500 dilutionLane 1 : CHRDL1 transfected 293T cell lysateLane 2 : 293T non-transfected cell lysate