Anti-Chromogranin A antibody
| Name | Anti-Chromogranin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15160 |
| Prices | $400.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human, Monkey |
| Antigen | Recombinant fragment from the C-terminal (Human) |
| Description | Rabbit Polyclonal |
| Gene | CHGA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab15160 at 1/250 staining human pancreas tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the antibody for 30 minutes at 25°C. An HRP conjugated rabbit polyclonal was used as the secondary.See Abreview
ab15160 (1/100) staining Chromogranin A in paraffin-embbeded mouse pancreas tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (10 minutes, 1.5% serum). An HRP-conjugated goat polyclonal was used as the secondary. For further experimental details please refer to abreview.See Abreview
ab15160 staining Chromogranin A in mouse gastric gland cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton ×100 and blocking with 10% serum for 45min at 200C was performed. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. A Texas Red ®-conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.See Abreview
ab15160 staining Chromogranin A in mouse colon tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formalin fixation before heat mediated antigen retrieval with 10mM citric acid buffer and then the sample was blocked for 45 minutes at 20°C. The primary antibody (1/100) was incubated with sample in 10% serum for 18 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution 1/200 as secondary antibody.See Abreview
Anti-Chromogranin A antibody (ab15160) at 1/100 dilution + Human Chromogranin A full length protein (ab85486) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Product References
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