All lanes : Anti-CISD1 antibody [2B3] (ab118027) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNALysates/proteins at 5 µg per lane.
ab118027, at 1/100 dilution, staining CISD1 in COS7 cells transiently transfected by pCMV6-ENTRY CISD1 by Immunofluorescence.
Flow cytometric Analysis of CISD1 staining in Jurkat cells, using ab118027 at 1/100 dilution(Red), compared to a nonspecific negative control antibody(Blue).