All lanes : Anti-CITED2 antibody (ab99335) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLane 2 : SW13 (Human adrenal adenocarcinoma) Whole Cell LysateLane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.