![Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/1000 dilution + Human brain tissue lysate - whole at 50 µgSecondaryHRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29100_ab82426-207011-ab82426wb.jpg)
Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/1000 dilution + Human brain tissue lysate - whole at 50 µgSecondaryHRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![All lanes : Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/2000 dilutionLane 1 : HeLa cell lysatesLane 2 : Ramos cell lysatesLane 3 : A431 cell lysatesLane 4 : 293T cell lysatesLane 5 : Jurkat cell lysatesLane 6 : PC12 cell lysates](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29101_CK1-epsilon-Primary-antibodies-ab82426-1.jpg)
All lanes : Anti-CK1 epsilon antibody [AF6C1] (ab82426) at 1/2000 dilutionLane 1 : HeLa cell lysatesLane 2 : Ramos cell lysatesLane 3 : A431 cell lysatesLane 4 : 293T cell lysatesLane 5 : Jurkat cell lysatesLane 6 : PC12 cell lysates
![Overlay histogram showing HeLa cells stained with ab82426 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82426, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29102_CK1-epsilon-Primary-antibodies-ab82426-2.jpg)
Overlay histogram showing HeLa cells stained with ab82426 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82426, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.