Anti-Claspin antibody
| Name | Anti-Claspin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3720 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB IHC-P |
| Species Reactivities | Human, Chimpanzee, Ape, Orangutan |
| Antigen | Synthetic peptide (Human) - which represented a portion of human Claspin encoded in part by exons 11, 12 and 13 |
| Description | Rabbit Polyclonal |
| Gene | CLSPN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Claspin with ab3720 at 1/1000 (1µg/ml). Detection: DAB.
ab3720 (1/200) staining Claspin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.See Abreview
ICC/IF image of ab2861 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2861, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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CDC6 interaction with ATR regulates activation of a replication checkpoint in - CDC6 interaction with ATR regulates activation of a replication checkpoint in
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ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand - ATM- and cell cycle-dependent regulation of ATR in response to DNA double-strand
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