Anti-Claudin 1 antibody

• Supplier: Abcam
• Catalog: ab15098
• Prices: $400.00
• Sizes: 500 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ICC/IF ICC/IF WB IP IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human, Dog, Monkey
• Antigen: Synthetic peptide (Human) (C terminal)
• Description: Rabbit Polyclonal
• Gene: CLDN1
• Conjugate: Unconjugated

Product images

Human skin stained with ab15098.

ab15098 staining Claudin 1 in mouse kidney tissue sections by IHC-P (paraformaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrival was by heat mediation in Citrate buffer (pH6). The sample was incubated with primary antibody (1/500 in blocking buffer) at 22°C for 16 hours. A Biotin-conjugated Donkey polyclonal to rabbit IgG (1/250) was used as secondary antibody.See Abreview

ab15098 (1µg/ml) staining Claudin 1 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ICC/IF image of ab15098 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Claudin 1 antibody (ab15098) at 1/25 dilution + HeLa cell lysate

Product References

Expressions of tight junction proteins Occludin and Claudin-1 are under the - Expressions of tight junction proteins Occludin and Claudin-1 are under the

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