Anti-CLCN3 antibody - Cytoplasmic domain

• Supplier: Abcam
• Catalog: ab86192
• Prices: $378.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P IHC-F
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide derived from the cytoplasmic domain of Rat CLCN3, conjugated to an immunogenic carrier protein
• Description: Rabbit Polyclonal
• Gene: CLCN3
• Conjugate: Unconjugated

Product images

ICC/IF image of ab86192 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86192, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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Zhang H, Li H, Yang L, Deng Z, Luo H, Ye D, Bai Z, Zhu L, Ye W, Wang L, Chen L. Sci Rep. 2013;3:2615.

Tamoxifen inhibits migration of estrogen receptor-negative hepatocellular - Tamoxifen inhibits migration of estrogen receptor-negative hepatocellular

Mao J, Yuan J, Wang L, Zhang H, Jin X, Zhu J, Li H, Xu B, Chen L. J Cell Physiol. 2013 May;228(5):991-1001.