CLCN3

Gene Symbol CLCN3
Entrez Gene 1182
Alt Symbol CLC3, ClC-3
Species Human
Gene Type protein-coding
Description chloride channel, voltage-sensitive 3
Other Description H(+)/Cl(-) exchange transporter 3|chloride channel 3|chloride channel protein 3|chloride transporter ClC-3
Swissprots Q86Z21 D3DP34 B7Z932 P51790 O14918 E9PB97 B9EGJ9
Accessions EAX04782 EAX04783 EAX04784 EAX04785 EAX04786 P51790 AA780395 AB019542 BAC54560 AF029346 AAB95161 AF086418 AF172729 AAD51034 AK127483 BAG54512 AK304362 BAH14168 AL117599 AM392951 CAL37829 BC026256 BC054877 AAH54877 BC058818 BC136510 AAI36511 BC146697 AAI46698 BP284718 BX647119 DA102505 X78520 CAA55280 XM_005262726 XP_005262783 XM_011531586 XP_011529888 NM_001243372 NP_001230301 NM_001243374 NP_001230303 NM_001829 NP_001820 NM_173872 NP_776297
Function Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. {ECO:0000269|PubMed:11967229}.
Subcellular Location Isoform 1: Membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Note=Isoform 1 is localized mainly in early and late endosomes.
Tissue Specificity Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level). {ECO:0000269|PubMed:10198195, ECO:0000269|PubMed:11967229, ECO:0000269|PubMed:12471024}.

CLCN3 (D8Y5Q) Rabbit mAb - 13359 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8Y5Q
Western blot analysis of extracts from various cell lines and tissues using CLCN3 (D8Y5Q) Rabbit mAb.

CLC-3 (K-17) - sc-17572 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CLC-3 (K-17): sc-17572. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane and cytoplasmic staining of glandular cells.

CLC-3 (B-21) - sc-133466 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
CLC-3 (B-21): sc-133466. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.

CLC-3/4/5 (H-4) - sc-390010 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CLC-3/4/5 (H-4): sc-390010. Western blot analysis of CLC-3/4/5 expression in T84 (A) and HT-29 (B) whole cell lysates.

CLC-3/4/5 (H-300) - sc-28762 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-CLCN3 antibody [S258-5] - ab134285 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S258-5
IHC image of CLCN3 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134285, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CLCN3 antibody - Cytoplasmic domain - ab86192 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab86192 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86192, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ClC-3 / CLCN3 Antibody (C-Terminus) - LS-C6125 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-3 / CLCN3 Antibody (C-Terminus) - LS-C6124 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ClC-3 / CLCN3 Antibody (aa29-236) - LS-C6126 from LifeSpan Bioscience

Host Chicken
Clonality Polyclonal

Anti-ClC-3 / CLCN3 Antibody (clone S258-5) - LS-C150481 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 700) - LS-C228693 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 655) - LS-C228694 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 680) - LS-C228692 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, APC) - LS-C228695 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Biotin) - LS-C228697 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, FITC) - LS-C228698 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, AP) - LS-C228696 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, PE-Atto 594) - LS-C228700 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, SA) - LS-C228702 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, RPE) - LS-C228701 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, PerCP) - LS-C228703 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 390) - LS-C229992 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 488) - LS-C229993 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5

Anti-ClC-3 / CLCN3 Antibody (aa98-115, clone S258-5, Atto 594) - LS-C229995 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone S258-5
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