All lanes : Anti-CLEC2A antibody (ab76711) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.