![Anti-CLEC4E antibody [AT16E3] (ab100846) at 1/1000 dilution + HeLa Cell Lysate at 35 µgSecondarygoat anti-mouse conjugated to HRP developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29634_CLEC4E-Primary-antibodies-ab100846-1.jpg)
Anti-CLEC4E antibody [AT16E3] (ab100846) at 1/1000 dilution + HeLa Cell Lysate at 35 µgSecondarygoat anti-mouse conjugated to HRP developed using the ECL technique
Immunofluorescence of Human HeLa cells stained with ab100846 (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
ab100846 staining CLEC4E in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.
![Overlay histogram showing HeLa cells stained with ab100846 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100846, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/29637_CLEC4E-Primary-antibodies-ab100846-4.jpg)
Overlay histogram showing HeLa cells stained with ab100846 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100846, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.