Anti-CLIC4 antibody [EPR14253]

• Supplier: Abcam
• Catalog: ab183043
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14253
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CLIC4 aa 1-100
• Description: Rabbit Monoclonal
• Gene: CLIC4
• Conjugate: Unconjugated

Product images

All lanes : Anti-CLIC4 antibody [EPR14253] (ab183043) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : 293 lysateLane 3 : Raji lysateLane 4 : HepG2 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunofluorescence analysis of HeLa cells (-20°C Acetone-fixed) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot analysis of immunoprecipitation pellet from Human Raji cell lysate immunoprecipitated using ab183043 at 1/50 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary antibody used at 1/1500 dilution.

Flow cytometric analysis of Raji cells (paraformaldehyde-fixed, 2%) labeling CLIC4 with ab183043 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

Immunofluorescence analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling CLIC4 with ab183043 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling CLIC4 with ab183043 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.