Anti-CLK3 antibody (ab88135) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg/mlSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Anti-CLK3 antibody (ab88135) at 1/500 dilution + Hela cell lysate at 25 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
All lanes : Anti-CLK3 antibody (ab88135) at 1/500 dilutionLane 1 : CLK3 transfected lysate 293T cell lineLane 2 : Non-transfected lysate 293T cell lineLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
ab88135 at 0.5µg/ml staining CLK3 in human placenta tissue.
ab88135 at 8µg/ml staining CLK3 in Hela cells.
All lanes : Anti-CLK3 antibody (ab88135) at 1/500 dilutionLane 1 : Non-transfected control Lane 2 : CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAiLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution