HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained with ab119065 at 1/100, and then analyzed by flow cytometry.
All lanes : Anti-CLPP antibody [1E2] (ab119065) at 1/500 dilutionLane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cells were transfected with the pCMV6-ENTRY CLPP cDNA for 48 hrs and lysedLysates/proteins at 5 µg per lane.
All lanes : Anti-CLPP antibody [1E2] (ab119065) at 1/500 dilutionLane 1 : HepG2 cell extractsLane 2 : Hela cell extractsLane 3 : HT29 cell extractsLane 4 : A549 cell extractsLane 5 : Cos7 cell extractsLane 6 : Jurkat cell extractsLane 7 : MDCK cell extractsLane 8 : PC12 cell extractsLane 9 : MCF7 cell extractsLysates/proteins at 35 µg per lane.