Chk1 siRNA (h): sc-29269. Western blot analysis of Chk1 expression in non-transfected control (A) and Chk1 siRNA transfected (B) HeLa cells. Blot probed with Chk1 (G-4): sc-8408. GAPDH (V-18): sc-20357 used as specificity and loading control.
Chk1 (G-4) PE: sc-8408 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG
2a: sc-2867.
Chk1 (G-4): sc-8408. Western blot analysis of Chk1 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.
Chk1 (G-4): sc-8408. Western blot analysis of Chk1 expression in non-transfected 293T: sc-117752 (A), mouse Chk1 transfected 293T: sc-119228 (B) and K-562 (C) whole cell lysates.