Lane 1 : MarkerLanes 2 - 4 : Anti-CNAP1 antibody (ab34338) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µgSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlas ab34338 staining CNAP1 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org