NCAPD2

Gene Symbol NCAPD2
Entrez Gene 9918
Alt Symbol CAP-D2, CNAP1, hCAP-D2
Species Human
Gene Type protein-coding
Description non-SMC condensin I complex, subunit D2
Other Description XCAP-D2 homolog|chromosome condensation related SMC associated protein 1|chromosome condensation-related SMC-associated protein 1|chromosome-associated protein D2|condensin complex subunit 1
Swissprots Q8N6U3 Q15021 D3DUR4
Accessions EAW88788 EAW88789 EAW88790 Q15021 AK022511 BAG51082 AK125155 BAC86066 AK128354 BAG54665 AK303310 BAG64379 AL548223 BC016913 AAH16913 BC028182 AAH28182 CN307514 D63880 BAA09930 DQ573571 NM_014865 NP_055680
Function Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. {ECO:0000269|PubMed:11136719}.
Subcellular Location Nucleus {ECO:0000269|PubMed:10958694}. Cytoplasm {ECO:0000269|PubMed:10958694}. Chromosome {ECO:0000269|PubMed:10958694}. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

hCAP-D2 (D-3) - sc-166878 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hCAP-D2 (D-3): sc-166878. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

hCAP-D2 (E-6) - sc-398950 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
hCAP-D2 (E-6): sc-398950. Western blot analysis of hCAP-D2 expression in Ramos (A), K-562 (B), NIH/3T3 (C) and U-251-MG (D) whole cell lysates.

hCAP-D2 (F-19) - sc-49961 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hCAP-D2 (F-19): sc-49961. Western blot analysis of hCAP-D2 expression in HeLa whole cell lysate.

hCAP-D2 (H-300) - sc-99217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
hCAP-D2 (H-300): sc-99217. Western blot analysis of hCAP-D2 expression in Jurkat (A), K-562 (B), Ramos (C) and NIH/3T3 (D) whole cell lysates.

hCAP-D2 (N-20) - sc-49962 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
hCAP-D2 (N-20): sc-49962. Western blot analysis of hCAP-D2 expression in HeLa whole cell lysate.

hCAP-D2 (294.1) - sc-101012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
hCAP-D2 (294.1): sc-101012. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic localization.

Anti-CNAP1 antibody - ab56885 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56885 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56885, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CNAP1 antibody - ab34338 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : MarkerLanes 2 - 4 : Anti-CNAP1 antibody (ab34338) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µgSecondaryLanes 2 - 4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-CNAP1 antibody - ab34307 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CNAP1 antibody (ab34307) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgSecondaryIR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-CNAP1 antibody [EPR9220] - ab137075 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9220
All lanes : Anti-CNAP1 antibody [EPR9220] (ab137075) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CNAP1 antibody - C-terminal - ab198019 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CNAP1 antibody - C-terminal (ab198019) at 1/425 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLysates/proteins at 40 µg per lane.

Anti-NCAPD2 Antibody (aa1279-1328) - LS-C82492 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Jurkat cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 12%.

Anti-NCAPD2 Antibody (clone 4C12) - LS-C133608 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C12
Immunoperoxidase of monoclonal antibody to CNAP1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Anti-NCAPD2 Antibody (C-Terminus) - LS-C77688 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NCAPD2 Antibody (C-Terminus) - LS-C286009 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NCAPD2 Antibody (aa1240-1339, Azide-free) - LS-C330363 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

CNAP1 Antibody (4C12) - H00009918-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C12
Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.

CNAP1 Antibody - NB100-1570 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CNAP1 Antibody [NB100-1570] - Detection of Human CAP-D2 on HeLa whole cell lysate using NB100-1570.

CNAP1 Antibody - H00009918-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

CNAP1 Antibody (4C9) - H00009918-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C9

CAPD2 Polyclonal Antibody - bs-7718R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-CNAP1 antibody produced in mouse - WH0009918M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C12
<B>Immunoblotting</B><BR/>Monoclonal Anti-CNAP1, <B>Cat. No. WH0009918M1</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~157.1 kDa using HeLa lysate.

Monoclonal Anti-CNAP1 antibody produced in mouse - SAB1404764 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4C9

Anti-NCAPD2 antibody produced in rabbit - HPA037363 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NCAPD2: <B>Cat. No. HPA037363</B>: Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.

Anti-NCAPD2 antibody produced in rabbit - HPA036947 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NCAPD2: <B>Cat. No. HPA036947</B>: Immunohistochemical staining of human tonsil shows strong cytoplasmic and nuclear positivity in reaction center cells.
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