All lanes : Anti-CNTN4 antibody (ab93450) at 1 µg/mlLane 1 : Mouse Hippocampus Tissue LysateLane 2 : Rat Olfactory Bulb LysateLane 3 : E14 Mouse Embryo Brain Tissue LysateLane 4 : E16 Ms Embryo Brain Tissue Lysate Lane 5 : E18 Ms Embryo Brain Tissue LysateLane 6 : Brain (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 150 kDa (why is the actual band size different from the predicted?)Additional bands at : 56 kDa (possible isoform).Exposure time : 12 minutes