IHC image of Collagen II staining in Human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85266, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
All lanes : Anti-Collagen II antibody (ab85266) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 2 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.