Anti-Connexin 43 / GJA1 antibody [4E6.2]
| Name | Anti-Connexin 43 / GJA1 antibody [4E6.2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79010 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4E6.2 |
| Applications | FC WB ELISA IHC-F ICC/IF |
| Species Reactivities | Mouse, Rat, Dog, Human, Pig, Sheep, Rabbit, Guinea Pig, Bovine, Hamster |
| Antigen | Synthetic peptide: GPLSPSKDCGSPKYAYFNG , corresponding to amino acids 252-270 of Mouse Connexin 43/ GJA1 Run BLAST with Run BLAST with |
| Blocking Peptide | Connexin 43 / GJA1 peptide |
| Description | Mouse Monoclonal |
| Gene | GJA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat anti-mouse HRP](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/828_Connexin-43-GJA1-Primary-antibodies-ab79010-3.gif)
Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat anti-mouse HRP
![All lanes : Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateSecondaryGoat anti-mouse HRP](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/829_Connexin-43-GJA1-Primary-antibodies-ab79010-5.gif)
All lanes : Anti-Connexin 43 / GJA1 antibody [4E6.2] (ab79010) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateSecondaryGoat anti-mouse HRP
![Overlay histogram showing SH-SY5Y cells stained with ab79010 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79010, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/830_Connexin-43-GJA1-Primary-antibodies-ab79010-6.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab79010 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79010, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Effects of doxycycline on cx43 distribution and cardiac arrhythmia susceptibility - Effects of doxycycline on cx43 distribution and cardiac arrhythmia susceptibility
Fana XZ, Zhu HJ, Wu X, Yan J, Xu J, Wang DG. Iran J Pharm Res. 2014 Spring;13(2):613-21.
Bioactive 3D cell culture system minimizes cellular stress and maintains the in - Bioactive 3D cell culture system minimizes cellular stress and maintains the in
Puschmann TB, Zanden C, De Pablo Y, Kirchhoff F, Pekna M, Liu J, Pekny M. Glia. 2013 Mar;61(3):432-40.