Anti-COPS3 antibody [EPR3127]
| Name | Anti-COPS3 antibody [EPR3127] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79698 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3127 |
| Applications | WB IP FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human) from the C-terminus |
| Description | Rabbit Monoclonal |
| Gene | COPS3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-COPS3 antibody [EPR3127] (ab79698) at 1/20000 dilutionLane 1 : HT-29 cell lysateLane 2 : HeLa cell lysateLane 3 : SKBR-3 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/930_COPS3-Primary-antibodies-ab79698-1.jpg)
All lanes : Anti-COPS3 antibody [EPR3127] (ab79698) at 1/20000 dilutionLane 1 : HT-29 cell lysateLane 2 : HeLa cell lysateLane 3 : SKBR-3 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescent staining of HeLa cells using ab79698 at 1/250 dilution.
Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79698, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Dynamic regulation of the COP9 signalosome in response to DNA damage. - Dynamic regulation of the COP9 signalosome in response to DNA damage.
Fuzesi-Levi MG, Ben-Nissan G, Bianchi E, Zhou H, Deery MJ, Lilley KS, Levin Y, Sharon M. Mol Cell Biol. 2014 Mar;34(6):1066-76.
The COP9 signalosome interacts with and regulates interferon regulatory factor 5 - The COP9 signalosome interacts with and regulates interferon regulatory factor 5
Korczeniewska J, Barnes BJ. Mol Cell Biol. 2013 Mar;33(6):1124-38.