Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 minutes), labeling COPS3 with ab199637 at 1/100 dilution.
All lanes : Anti-COPS3 antibody - C-terminal (ab199637) at 1/1000 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from HepG2 cellsLysates/proteins at 12.5 µg per lane.
All lanes : Anti-COPS3 antibody - C-terminal (ab199637) at 1/1000 dilutionLane 1 : HT-29 cell line lysateLane 2 : HeLa cell line lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-rabbit IgG H&L(HRP) at 1/5000 dilution