Anti-COX2 / Cyclooxygenase 2 antibody
| Name | Anti-COX2 / Cyclooxygenase 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15191 |
| Prices | $400.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF ELISA IP IHC-P WB |
| Species Reactivities | Mouse, Rat, Human, Hamster |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)(Rat) |
| Description | Rabbit Polyclonal |
| Gene | PTGS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab15191 staining COX2 in HepG2 cells treated with sinomenine hydrochloride (ab141190), by ICC/IF. Decrease of COX2 expression correlates with increased concentration of sinomenine hydrochloride, as described in literature.The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141190 (sinomenine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15191 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
![Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/1357_COX2-Cyclooxygenase-2-Primary-antibodies-ab15191-11.jpg)
Standard Curve for COX2 / Cyclooxygenase 2 (Analyte: ab58868) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS66] to COX2 / Cyclooxygenase 2 (ab90345) at 1ug/ml and Detector Antibody Rabbit polyclonal to COX2 / Cyclooxygenase 2 (ab15191) at 0.5ug/ml
ab15191 staining COX2 / Cyclooxygenase in human breast carcinoma by Immunohistochemistry (FFPE-sections).
ab15191 staining mouse brain sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/2000 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit IgG antibody, diluted 1/300, was used as the secondary. Sections were preblocked in an Avidin-Biotin kit to mask endogenous biotinSee Abreview
ab15191 staining adult rat hippocampus tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval (in citric acid, pH6, 10mM) prior to blocking in 1% BSA for 10 minutes at RT. The primary anitbody was diluted 1/400 and incubated with the sample for 2 hours. A biotinylated goat anti-rabbit antibody, diluted 1/300 was used as the secondary.Clear cytoplasmic staining in a subset of neurones in the dentate gyrus of a sagittal section of whole rat brain can be seen. In other areas of this section, there are, additionally, small cells and their processes that are positive: they may be inter-neurones or microglia: it would be neccessary to carry out double-immunostaining to confirm this.See Abreview
ab15191 staining tissue sections of arteriosclerotic plaque in mouse aorta by IHC-Fr. Sections were acetone fixed and blocked in 1% serum for 10 minutes at 20°C prior to incubation with the primary antibody (diluted 1/500) for 1 hour at 20°C. A biotinylated donkey anti-rabbit antibody (diluted 1/500) was used as the secondary. See Abreview
developed using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
ab15191 staining COX2 / Cyclooxygenase 2 in Mouse liver tissue sections by IHC-Fr.Cells were fixed with acetone and blocked with 10% Serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% Triton x-100) for 13 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/1000) was used as the secondary antibody.See Abreview
Product References
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Disruption of cyclooxygenase-2 prevents downregulation of cortical AQP2 and AQP3 - Disruption of cyclooxygenase-2 prevents downregulation of cortical AQP2 and AQP3
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