Anti-Creatine kinase B type antibody [EPR3926]

• Supplier: Abcam
• Catalog: ab108388
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3926
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human Creatine Kinase BB
• Description: Rabbit Monoclonal
• Gene: CKB
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab108388 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108388, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Creatine kinase B type antibody [EPR3926] (ab108388) at 1/50000 dilutionLane 1 : 293T cell lysateLane 2 : SH SY5Y cell lysateLane 3 : Y79 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue.

Product References

Control of creatine metabolism by HIF is an endogenous mechanism of barrier - Control of creatine metabolism by HIF is an endogenous mechanism of barrier

Glover LE, Bowers BE, Saeedi B, Ehrentraut SF, Campbell EL, Bayless AJ, Dobrinskikh E, Kendrick AA, Kelly CJ, Burgess A, Miller L, Kominsky DJ, Jedlicka P, Colgan SP. Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19820-5. doi: