CKB

Gene Symbol CKB
Entrez Gene 1152
Alt Symbol B-CK, BCK, CKBB, HEL-211, HEL-S-29
Species Human
Gene Type protein-coding
Description creatine kinase, brain
Other Description creatine kinase B chain|creatine kinase B-type|creatine kinase-B|epididymis luminal protein 211|epididymis secretory protein Li 29
Swissprots B2R5R4 A8K236 Q6FG40 Q2LE07 Q9UC66 P12277
Accessions AAA52024 AAC31758 ABC67465 CAA33389 EAW81820 EAW81821 EAW81822 P12277 AK290101 BAF82790 AK298198 BAG60468 AK312282 BAG35211 BC001190 AAH01190 BC004914 AAH04914 BC008323 AAH08323 BC010002 AAH10002 BC019259 AAH19259 BC019281 AAH19281 BG720434 BM684665 BP213119 CR542268 CAG47064 EU794629 ACJ13683 FJ224337 ACI46029 JF432538 ADZ15755 L47647 AAA76852 M16364 AAA76850 M16451 AAA76851 NM_001823 NP_001814
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Subcellular Location Cytoplasm.
Top Pathways Arginine and proline metabolism

creatine kinase-B (B-9) - sc-373686 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-B (B-9): sc-373686. Western blot analysis of creatine kinase-B expression in NCI-H1688 (A) and SH-SY5Y (B) whole cell lysates.

creatine kinase-B (C-17) - sc-15160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

creatine kinase-B (G-6) - sc-374072 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-B (G-6): sc-374072. Western blot analysis of creatine kinase-B expression in SH-SY5Y whole cell lysate.

creatine kinase-B (N-20) - sc-15157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
creatine kinase-B (N-20): sc-15157. Western blot analysis of creatine kinase-B expression in NCI-H1688 (A) and SH-SY5Y (B) whole cell lysates and mouse brain tissue extract (C).

creatine kinase-M/B (CK1) - sc-56896 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-M/B (CK1): sc-56896. Western blot analysis of creatine kinase-M/B expression in SH-SY5Y (A) and HeLa (B) whole cell lysates.

creatine kinase-M/B (CK-10) - sc-51843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-M/B (CK-10): sc-51843. Western blot analysis of human recombinant creatine kinase-M/B.

creatine kinase-M/B (BDI937) - sc-56895 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-M/B (BDI937): sc-56895. Western blot analysis of creatine kinase-M/B expression in SH-SY5Y (A) and HeLa (B) whole cell lysates.

creatine kinase-M/B (1.BB.105) - sc-70881 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
creatine kinase-M/B (1.BB.105): sc-70881. Western blot analysis of creatine kinase-M/B expression in SH-SY5Y (A) and HeLa (B) whole cell lysates.

Anti-Creatine kinase B type antibody - ab38211 from Abcam

Prices $391.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Creatine kinase B type antibody (ab38211) at 1/100 dilutionLane 1 : Y79 cell lysate Lane 2 : Mouse colon tissue lysate

Anti-Creatine kinase B type antibody - ab38212 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Creatine kinase B type antibody (ab38212) at 1/100 dilutionLane 1 : Mouse colon tissue lysateLane 2 : Y79 cell lysate

Anti-Creatine kinase B type antibody - ab48006 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Creatine kinase B type antibody (ab48006) at 0.1 µg/ml + Human Brain lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-Creatine kinase B type antibody - ab2117 from Abcam

Prices $368.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal

Anti-Creatine kinase B type antibody - ab6496 from Abcam

Prices $368.00
Sizes 500 µl
Host Goat
Clonality Polyclonal

Anti-Creatine kinase B type antibody [3A6] - ab71944 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A6
All lanes : Anti-Creatine kinase B type antibody [3A6] (ab71944) at 1/2000 dilutionLane 1 : Recombinant CK-MM protein isolated from E. coliLane 2 : Recombinant CK-BB protein isolated from E. coliLane 3 : Recombinant CK-MB protein isolated from E. coliLane 4 : Human CK-MB protein

Anti-Creatine kinase B type antibody [893CT29.1.1] - ab180040 from Abcam

Prices $376.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 893CT29.1.1
All lanes : Anti-Creatine kinase B type antibody [893CT29.1.1] (ab180040) at 1/1000 dilutionLane 1 : MDA-MB453 cell lysateLane 2 : 293 cell lysateLane 3 : Y-79 cell lysateLane 4 : Mouse stomach tissue lysateLane 5 : Mouse brain tissue lysateLysates/proteins at 35 µg per lane.

Anti-Creatine kinase B type antibody (Biotin) - ab48651 from Abcam

Prices $370.00
Sizes 150 µg
Host Rabbit
Clonality Polyclonal

Anti-Creatine kinase B type antibody [EPR3926] - ab108388 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3926
Overlay histogram showing SH-SY5Y cells stained with ab108388 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108388, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Creatine kinase B type antibody [EPR3926] (HRP) - ab197066 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3926
All lanes : Anti-Creatine kinase B type antibody [EPR3926] (HRP) (ab197066) at 1/5000 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 3 : Y79 (Human retinoblastoma cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Creatine kinase B type antibody [EPR3927] - ab92452 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3927
All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilutionLane 1 : SHSY-5Y cell lysateLane 2 : HeLa cell lysateLane 3 : Y-79 cell lysateLane 4 : SW840 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Creatine Kinase MB antibody - ab31832 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Image from Hurd TR et al, J Biol Chem. 2008 Sep 5;283(36):24801-15. Epub 2008 Jul 8, Fig S1.

Anti-Creatine Kinase MB antibody - ab30936 from Abcam

Prices $370.00
Sizes 500 µg
Host Chicken
Clonality Polyclonal

Anti-Creatine Kinase MB antibody [46A1] - ab72002 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 46A1
IHC image of Creatine Kinase MB  staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72002, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Creatine Kinase MB antibody [BDI937] - ab19603 from Abcam

Prices $372.00
Sizes 500 µg
Host Mouse
Clonality Monoclonal
Clone BDI937

Anti-Creatine Kinase MB antibody [CK-10] - ab10045 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone CK-10

Anti-Creatine Kinase MB antibody [CK1] - ab404 from Abcam

Prices $372.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone CK1
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