Anti-Creatine kinase B type antibody [EPR3927]

Name	Anti-Creatine kinase B type antibody [EPR3927]
Supplier	Abcam
Catalog	ab92452
Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR3927
Applications	WB IHC-P FC ICC/IF ICC/IF
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide corresponding to residues near the C-terminus of Human Creatine Kinase B type
Description	Rabbit Monoclonal
Gene	CKB
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilutionLane 1 : SHSY-5Y cell lysateLane 2 : HeLa cell lysateLane 3 : Y-79 cell lysateLane 4 : SW840 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Paraffin embedded Human glioma tissue labelled with ab92452 at 1/100 dilution.

Immunofluorescence analysis of HeLa cells labelled with ab92452 at 1/100 dilution.

Overlay histogram showing HeLa cells stained with ab92452 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92452, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.