Anti-Creatine Kinase MB antibody [46A1]

• Supplier: Abcam
• Catalog: ab72002
• Prices: $378.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 46A1
• Applications: WB ELISA IHC-P
• Species Reactivities: Human
• Antigen: Recombinant human Creatine Kinase MB protein purified from E
• Description: Mouse Monoclonal
• Gene: CKB
• Conjugate: Unconjugated

Product images

IHC image of Creatine Kinase MB  staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72002, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Creatine Kinase MB antibody [46A1] (ab72002) at 1/2000 dilutionLane 1 : Recombinant human plasma Creatine Kinase MM protein isolated from E. coliLane 2 : Recombinant human plasma Creatine Kinase BB protein isolated from E. coliLane 3 : Recombinant human plasma Creatine Kinase MB protein isolated from E. coliLane 4 : Human plasma Creatine Kinase MB protein