Anti-CREM antibody
| Name | Anti-CREM antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54625 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | FC WB |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: ATGDMPTYQI RAPTAALPQG VVMAASPGSL HSPQQLAEEA TRKRELRLMK NREAAKECRR RKKEYVKCLE SRVAVLEVQN KKLIEELETL KDICSPKTDY , corresponding to amino acids 201-301 of Human CREM Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | CREM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab54625 CREM antibody at 1ug/ml.
![Overlay histogram showing HeLa cells stained with ab54625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54625, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/2230_CREM-Primary-antibodies-ab54625-1.jpg)
Overlay histogram showing HeLa cells stained with ab54625 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54625, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.